Coding

Part:BBa_K1867020:Design

Designed by: Shien Yang Lee   Group: iGEM15_NYU-AD   (2015-09-16)


LldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 716
    Illegal BamHI site found at 755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to sequence optimize to remove Biobrick restriction sites from coding sequence


Source

Coding sequence from Ecocyc database, accessed at: http://biocyc.org/ECOLI/sequence?object=EG11963


References